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Characterization of hydrocarbon degrading microorganisms from Glycine max and Zea mays phytoremediated crude oil contami
    Younsang Cho | 2022.05.09
Microbe-plant partnership in phytoremediation involves a synergistic interaction that leads to degradation of contaminants. The identification and
characterization of these microorganisms is fundamental in environmental management. This study is aimed at investigating the influence of Glycine
max and Zea mays on microbial make-up and differentiation of soil bacterial and fungal isolates in crude oil contaminated soil. We employed
conventional technique of microbial isolation and gene sequencing to evaluate the microbial composition in crude oil contaminated soil. The
microorganisms were isolated from crude oil contaminated soil (0%, 4%, 8%) and were identified using 16S rRNA gene (for bacteria) and Internal
Transcribed Spacer (ITS) gene (for fungi). We observed a change in the microbial cell density with respect to treatment conditions implying a shift
in microbial dynamics to total hydrocarbon utilizing bacteria as the dominant microbes. The sequence data revealed five bacteria strain; Klebsiella
aerogenes strain 77, Klebsiella aerogenes strain UISO178, Salmonella enterica strain ABUH7, Klebsiella aerogenes strain M242 and Enterobacter sp.
NCCP-607 and three fungi strains; Galactomyces geotrichum strain CBS, Aspergillus niger strain YMCHA73 and Trichoderma virens isolate A701.
Annotation analysis using FGENESB and gene scan revealed proteins involved in various metabolic processes and hydrocarbon utilization. GHOSTKOLA
output revealed several genetic elements and pathways such as DnaA, PYG, mrcA, environmental, cellular and genetic information processing and
degradation enhancers. Our findings show that G. max and Z. mays in association with bacteria can enhance ecosystem restoration of crude oil
contaminated soil.

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